Synthetic polymeric scaffolds design and fabrication for wound healing treatment

Authors

  • Gabriela Rodriguez Laboratorio de medios e interfases
  • María Lourdes Gonzales Laboratorio de medios e interfases
  • Silvia Noemí Kozuszko Cátedra de Anatomía y fisiología patológicas
  • Juan Carlos Valdez Cátedra de Inmunología
  • Andrea Paola Rodriguez Laboratorio de medios e interfases

Abstract

Ulcers are a type of chronic wounds that are very susceptible to microbial infections of bacteria that produce biofilm, which makes them refractory to antibiotic and antiseptic treatment. It has been observed that treatment with Lactobacilus plantarum supernatant (SNLP) in venous ulcers, reduces wound healing time, significantly decreasing bacterial load, so it could be a good alternative as treatment for different types of chronic wounds. Polycaprolactone (PCL) is a biocompatible and biodegradable polymer, with excellent mechanical properties, whose effectiveness in dermatological applications has been widely demonstrated. In addition, with the electrospinning technique, PCL fibers can be created in micro and nanometric scale with a certain degree of porosity, very useful for different biomedical applications. Therefore, the purpose of this work is to manufacture polymeric nanomembranes incorporating the supernatant of Lactobacilus plantarum, as an antibiotic and healing substance to PCL nanofibers by means of the immersion technique. The method of manufacturing PCL nanomembranes was optimized by electrospinning using various polymeric supports. Different immersion methods were tested for supernatant incorporation, controlled release of the same was studied and its morphology was observed in a Scanning Microscope. The 10% concentration PCL nanomembranes are made up of uniform nanofibers of random distribution, with an average diameter of 169.06 ± 32.28 nm and an average pore size of 1266.74 ± 292.55 nm. According to these results, fiber morphology was adequate for encapsulation. On the other hand, these scaffolds show antimicrobial activity, however, SNLP release was almost instantaneous, so, we conclude that it is not a suitable biomaterial as a possible treatment for chronic wounds. Therefore, it is necessary to review the technique to incorporate and controlled release of this substance and apply coaxial electrospinning for hollow nanofibers filled manufacture with this bioactive substance as an alternative technique.

Author Biographies

Gabriela Rodriguez, Laboratorio de medios e interfases

Departamento de Bioingeniería, Universidad Nacional de Tucumán, INSIBIO-Consejo Nacional de Investigaciones Científicas y Técnicas, Tucumán, Argentina

María Lourdes Gonzales, Laboratorio de medios e interfases

Departamento de Bioingeniería, Facultad de Ciencias Exactas, Universidad Nacional de Tucumán

Silvia Noemí Kozuszko, Cátedra de Anatomía y fisiología patológicas

Facultad de Odontología, UNIVERSIDAD NACIONAL DE TUCUMAN

Juan Carlos Valdez, Cátedra de Inmunología

Facultad de Bioquímica, Quí́mica, Farmacia y Biotecnología, Instituto de Microbiología, Universidad Nacional de Tucumán, Tucumán, Argentina. .

Andrea Paola Rodriguez, Laboratorio de medios e interfases

Departamento de Bioingeniería, Universidad Nacional de Tucumán, INSIBIO-Consejo Nacional de Investigaciones Científicas y Técnicas, Tucumán, Argentina. 

Downloads

Published

06/01/2021

Issue

Section

Scientific articles